Females of white-eared opossum Didelphis albiventris (Lund, 1841) from natural population of the "cerrado", State of Minas Gerais, Brazil, present a seasonal reproductive activity. Young-pouches were found in females captured from August to March. During the months of April to July youngs were not present in the pouch. The only two females captured in July showed pregnant uterus. All females collected in August and September were litter positive. The average number of individuais per litter was 7.04 ± 1.9.